Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA

Table 3 Copy number and Ct values of 8 clinical HBV serum samples detected by optimized primers, TaqMan probe from S region and NaOH lysis method (mean ± SD)

Sample	Ct		Copy number /mL
	Mean ± SD	CV (%)	Mean ± SD	CV (%)
1	25.4 ± 0.24	0.9	1.4 × 109± 2.38 × 108	17.0
2	31.9 ± 0.20	0.6	1.4 × 107± 1.87 × 106	13.4
3	31.5 ± 0.52	1.7	1.8 × 107± 2.32 × 106	12.9
4	27.3 ± 0.14	0.5	3.7 × 108± 3.73 × 107	10.0
5	34.0 ± 0.27	8.1	6.4 × 106± 6.08 × 105	9.5
6	32.3 ± 0.52	0.2	1.1 × 107± 4.43 × 106	4.0
7	27.0 ± 0.23	0.8	4.6 × 108± 7.22 × 106	15.7
8	33.9 ± 0.12	0.4	3.1 × 106± 2.76 × 105	8.9

CV: Coefficient variation.