Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA

Table 2 Copy number and Ct values of HBV serum samples detected by six different extraction methods (mean ± SD)

Method	Copy number/mL and Ct value
	Sample A	Sample B	Sample C
Guanidinium isothiocyanate	1.41 × 105± 1.23 × 104	1.92 × 105± 1.70 × 104	3.41 × 104± 3.65 × 103
	31.3 ± 0.16	30.9 ± 0.07	33.3 ± 1.59
Proteinase K	2.99 × 105± 2.88 × 104	2.21 × 105± 2.97 × 104	3.59 × 105± 5.10 × 104
	30.5 ± 0.19	30.3 ± 0.38	29.6 ± 0.38
NaI	2.51 × 105± 2.91 × 104	2.12 × 105± 3.01 × 104	9.12 × 104± 9.65 × 103
	30.7 ± 0.17	30.5 ± 0.66	31.4 ± 0.14
NaOH lysis	3.49 × 108± 2.72 × 107	2.08 × 106± 2.80 × 105	4.40 × 107± 2.06 × 106
	19.9 ± 0.18	30.0 ± 0.52	26.2 ± 0.78
Alkaline lysis	N/ N	N/ N	N/ N
Boiling	2.14 × 108± 2.16 × 107	1.83 × 106± 1.05 × 105	1.48 × 107± 1.22 × 106
	23.9 ± 0.10	30.1 ± 0.69	27.4 ± 0.94

N: Negative.