Elicitation of strong immune responses by a DNA vaccine expressing a secreted form of hepatitis C virus envelope protein E2 in murine and porcine animal models

Figure 4 Cytotoxic T lymphocyte (CTL) response to HCV E2 vaccines in mice. Murine splenocytes isolated from groups of mice two weeks after final immunization were pooled and stimulated with feeder cells infected with recombinant vaccinia virus expressing HCV E2 protein. The cytotoxic activity was determined by measuring the released lactate dehydrogenase (LDH) by the target cells after incubation with the effectors at different ratios.