Elicitation of strong immune responses by a DNA vaccine expressing a secreted form of hepatitis C virus envelope protein E2 in murine and porcine animal models

Figure 3 Cell-mediated immune responses to HCV E2 vaccines in mice. Murine splenocytes were isolated 2 wk after last immunization and were re-stimulated with E2 protein. Cell proliferation was analyzed by MTT assay (A). (P < 0.0001 vs saline; P < 0.05, P < 0.01 vs media). The number of interferon-γ secreting cells was determined by ELISPOT assay (B). (P < 0.05 vs plasmid; P < 0.0001 vs saline).