Viral Hepatitis
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Nov 28, 2006; 12(44): 7118-7125
Published online Nov 28, 2006. doi: 10.3748/wjg.v12.i44.7118
Figure 5
Figure 5 ELISPOT assay: Assessment at the individual single cell level of the number of mIFN-γ-secreting cells as means of CD8 T cell function. Spleen cells were stimulated with recombinant HCV-NS5 protein at 0.1 and 1 µg/mL, and the number of spot forming units/106 PBMC was counted after 24 h incubation period (n = 10 mice/group). Note that lymphocytes were not restimulated in NS5-expressing cells.