Genetic vaccination with Flt3-L and GM-CSF as adjuvants: Enhancement of cellular and humoral immune responses that results in protective immunity in a murine model of hepatitis C virus infection

Figure 2 Expression of Flt3-L in transiently transfected HuH-7 cells: (A) Detection of mouse Flt3-L on a 150 g/L SDS-PAGE using an anti-His-Tag antibody. Different glycosylation forms of Flt3-L in cell were detected in cell lysates and supernatants. Lane 1 and 3: mock-transfected cells; lane 2 and 4: Flt3-L-transfected cells; lane 1 and 2: supernatants after transient transfection; lane 3 and 4: cell lysates. (B) To confirm these results, supernatants and cell lysates were incubated by an Flt3-L-specific ELISA. Again, secretion levels into the supernatant were more than 20-fold higher compared to levels seen in the cell compartment.