Hypermethylation and expression regulation of secreted frizzled-related protein genes in colorectal tumor

Figure 4 A: Hypermethylation of sFRP genes in colorectal cancer cell lines; B: The mRNA expression level of sFRP genes in colorectal cancer cell lines before and after demethylation treatment. Three cell lines all received 4 different treatments: never treated (-), low-dose DAC (1), TSA (2), high-dose DAC (3), DAC + TSA (4); C: The sFRP1 gene expression index (EI) in three cell lines after demethylation treatment, low-dose DAC (1), high-dose DAC (3), DAC+TSA (4). ^bP < 0.01 vs 1, ^dP < 0.01 vs 3, ^aP < 0.05 vs 3.