Promoted differentiation of cynomolgus monkey ES cells into hepatocyte-like cells by co-culture with mouse fetal liver-derived cells

Figure 4 Characterization of MFLCs. A: Optical microscope image of MFLCs (× 100). MFLCs were prepared from E14.0 mouse livers and cultured for 48 h. After the floating cell fraction was discarded from the culture, resting adherent cells were further cultured until semi-confluent. MFLCs showed various morphologies, including cuboidal and stellate-shaped cells; B: RT-PCR analysis of MFLCs. AFP, desmin, and vimentin were expressed, whereas ALB was not. MAL: Mouse adult liver cells.