Promoted differentiation of cynomolgus monkey ES cells into hepatocyte-like cells by co-culture with mouse fetal liver-derived cells

Figure 3 RT-PCR analysis of GF-induced differentiation of cES cells. AFP mRNA expression was not detected following a 3-d treatment with RA and LIF, though it was detected on d 8, and at the end of 5 d of treatment with FGF2, FGF4, and HGF. ALB and HNF4α were detected after the differentiating cES cells were exposed to OSM and Dex. Although ALB and HNF4α were strongly detected on d 21 and 28, no expression of CYP7A1 was observed. Oct3/4 mRNA expression remained up to d 28.