Promoted differentiation of cynomolgus monkey ES cells into hepatocyte-like cells by co-culture with mouse fetal liver-derived cells

Figure 2 RT-PCR analysis of spontaneous differentiation of cES cells. AFP mRNA expression was not observed prior to d 14, while ALB was detected on d 21. On d 28, ALB and HNF4α expression became detectable. CYP7A1 was not detected throughout the experimental period, while Oct3/4 was distinctly detected at all time points.