Inhibition of hepatic tumor cell proliferation in vitro and tumor growth in vivo by taltobulin, a synthetic analogue of the tripeptide hemiasterlin

Figure 4 Microtubule (MT) structure assessed by immunofluorescene microscopy with a specific α-tubulin antibody. A and B: Untreated Hep3B and HepG2 cells showed dense and complex MT network in the cytoplasm. Insets A and B represent cells after HTI-286 treatment at 3 nmol/L for two cell doubling times. Decreased cytoplasmic microtubule density and diffuse staining were observed. Magnification x 40 except for insets, which are magnified further to outline the morphological changes. C: MH cells treated with HTI-286 at 1 nmol/L for two cell doubling times showed multipolar spindles, indicating disruption of the microtubule network in mitotic cells.