Attenuation of dextran sodium sulphate induced colitis in matrix metalloproteinase-9 deficient mice

Figure 1 Gelatinase activity in colonic homogenates. A: Representative zymogram in wild-type (wt) and MMP-9-deficient (KO) animals on d 5 (DSS 5) and 12 (DSS 12) after induction of colitis (n = 9 in each group). Supernatant of HT1080 cells, human recombinants MMP-9 (rMMP9) and MMP-2 (rMMP2) were used as positive controls. Gelatinases with molecular weights of 62, 72 and 92 kDa corresponding to activated MMP-2, pro-MMP-2 and pro-MMP-9, respectively, were detected. Quantitative data of pro-MMP-9 and total MMP-2 (pro-MMP-2 and MMP-2) (^aP < 0.05 vs control) in wt (B) and MMP-9-deficient mice (C).