Effect of transforming growth factor-β1 on human intrahepatic cholangiocarcinoma cell growth

Figure 3 Effect of TGF-β1 on the cell growth and apoptosis of ICC cell lines. A: The cells were subjected to a ³H-thymidine incorporation assay in the absence or presence of TGF-β1. No mitoinhibitory effect of TGF-β1 was observed in the ICC cells. TGF-β1 stimulation augmented the DNA synthesis of TGF-β1 in the HuCCT1 and MEC cells. In contrast, TGF-β1 treatment elicited a strong mitoinhibitory effect in Mv1Lu; B: Stimulation with 1 ng/mL TGF-β1 for 48 h did not induce apoptosis of HuCCT1 cells in a TUNEL assay. In the experiment using Mv1Lu, however, stimulation with TGF-β1 at the same concentration for the same duration did elicit an apoptotic effect: ^aP < 0.05 vs absence of TGF-β1; C: HuCCT1 and Mv1Lu cells were treated with 1 ng/mL TGF-β1 for 48 h. The arrows denote apoptotic cells identified by the TUNEL method. Scale bar = 100 μm.