Hydrodynamics based transfection in normal and fibrotic rats

Figure 4 400 μg of pGL3-Control plasmid DNA were injected in the following variations: (1) HBT: 5. 25% (volume per animal weight) Ringer solution with plasmid DNA in 5 to 8 s; (2) HBT Ringer + moderate injection: 5.25% (volume per animal weight) Ringer solution in 5 to 8 s followed by 400 μg of plasmid DNA in 500 μL 10 or 30 m later; (3) moderate injection: 500 μL Ringer solution with 400 μg plasmid DNA. Twenty four hours post injection the liver was excised. 200 mg liver sample were then homogenized in reporter buffer and luciferase activity monitored (n = 5).