Hydrodynamics based transfection in normal and fibrotic rats

Figure 1 400 μg of pGL3-Control plasmid DNA was injected in 5. 25% (volume per animal weight) Ringer to healthy rats by the hydrodynamics based injection (5 to 8 s). 24 h later the liver was excised, the lobes minced and 200 mg samples of each of the four lobes homogenized in reporter lysis buffer and luciferase activity monitored (n = 10).