Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells

Figure 5 Real-time PCR analysis of the expression of mRNA for liver markers determined at established times in the transdifferentiation protocol of BMSC and ADSC. Expression of C/EBPβ (A) (liver-enriched transcription factor, that plays important roles in regulating the expression of hepatic genes), albumin (B and C) and HNF4α (D), were analysed in BMSC (bold symbols) and ADSC (clear symbols) at different time points of the differentiation protocol. Data are shown as the fold increase in the mRNA level compared to the undifferentiated cells (d 0 of the differentiation protocol), and were normalized by hPBGD. The agarose gel shows the ALB expression of BMSC (C). HNF4α: hepatocyte nuclear factor 4 alpha; C/EBPβ: CCAAT / enhancer binding protein beta; ALB: albumin. Data are the mean ± SE of 5 and 6 different cultures of BMSC and ADSC respectively. ^aP < 0.05; ^bP < 0.01; ^dP < 0.001.