Occurrence of cGMP/nitric oxide-sensitive store-operated calcium entry in fibroblasts and its effect on matrix metalloproteinase secretion

Figure 7 cGMP/KT5823 and SNAP/L-NAME affecting MMP release 3T3 cells via Tg-induced SOC. 3T3 cells were incubated in NPBS with 4 μmol/L thapsigargin for 2 min. Each kind of chemical reagents (2 mmol/L cGMP, 1 μmol/L KT5823, 100 μmol/L SNAP or 200 µmol/L L-NAME) was introduced 1 min prior to the measurement, then 2 mmol/L CaCl₂ was added into each medium and incubated for 16-18 h. The 72 kDa gelatinolytic band (MMP-2) was obtained in cells without chemical treatment (control) or treated with cGMP, KT5823, SNAP, L-NAME.