Occurrence of cGMP/nitric oxide-sensitive store-operated calcium entry in fibroblasts and its effect on matrix metalloproteinase secretion

Figure 5 Expression of MMP in pretreated and not pretreated 3T3 cells. A: RT-PCR results of MMP-2 mRNA in both cells (1: DL2000 marker; 2: MMP-2 cDNA of 3T3 cells; 3: MMP-2 cDNA of 8-Br-cGMP (2 mmol/L) pre-treated 3T3 cells; 4: β-actin cDNA of 3T3 cells; 5: β-actin cDNA of pre-treated 3T3 cells); B: RT-PCR results of MMP-9 mRNA in pretreated and not pretreated 3T3 cells (1: DL2000 marker; 2: MMP-9 cDNA of 3T3 cells; 3: MMP-9 cDNA of 8-Br-cGMP (2 mM) pre-treated 3T3cells; 4: β-actin cDNA of 3T3 cells; 5: β-actin cDNA of pre-treated 3T3 cells); C: Gelatin zymography showing constitutive secretion of MMP-2 (72 kDa) and MMP-9 (92 kDa) into serum-free media by pretreated and not pretreated 3T3 cells.