Occurrence of cGMP/nitric oxide-sensitive store-operated calcium entry in fibroblasts and its effect on matrix metalloproteinase secretion

Figure 4 Effect of SNAP and L-NAME on thapsigargin-induced Ca²⁺ influx. A: 3T3 cells were incubated in NPBS with 4 μmol/L thapsigargin for 2 min, then 2 mmol/L CaCl₂ was added into the medium; B: 3T3 cells were incubated in NPBS with 4 μmol/L thapsigargin for 2 min, 100 μmol/L SNAP or 200 μmol/L L-NAME was introduced 1 min prior to the measurement, then 2 mmol/L CaCl₂ was added into the medium; C:.Ca²⁺ entry fluorescence ratios were obtained in cells without chemical treatment (control) or treated with 100 μmol/L SNAP or 200 μmol/L L-NAME. The values are the mean ± SE (n = 4-6).