Occurrence of cGMP/nitric oxide-sensitive store-operated calcium entry in fibroblasts and its effect on matrix metalloproteinase secretion

Figure 3 Effect of 8-bromo-cGMP and KT5823 on thapsigargin-induced Ca²⁺ influx. A: 3T3 cells were incubated in NPBS with 4 μmol/L thapsigargin for 2 min, then 2 mmol/L CaCl₂ was added into the medium; B: 3T3 cells were incubated in NPBS with 4 μmol/L thapsigargin for 2 min, 8-bromo-cGMP(2 mmol/L) or KT5823 (1 μmol/L) was introduced 1 min prior to the measurement, then 2 mmol/L CaCl₂ was added into the medium; C: Effects of cGMP and KT5823 on thapsigargin-induced Ca²⁺ influx.. The Ca²⁺ entry fluorescence ratios were obtained in cells without chemical treatment (control) or treated with 2 mmol/L 8-Br-cGMP (cGMP) or 1 μmol/L KT5823. The cells were incubated in NPBS with 4 μmol/L thapsigargin for 2 min prior to the chemical treatment. One mmol/L CaCl₂ was introduced to the medium to obtain the fluorescent signal of Ca²⁺ entry. The values are the mean ± SE (n = 4-6).