Differential reactivity of mouse monoclonal anti-HBs antibodies with recombinant mutant HBs antigens

Table 4 Reactivity patterns of monoclonal anti-HBs antibodies with expressed HBs antigen mutants

Monoclonal antibody	GlyD	GlyY	91-4696	SA6	SA7	1056	PA17D	M5	BA2.4	SA4	BA3.2
34D7-S3	++	++	++	++	++	+	-	-	+	+	-
33D7-S4	++	++	++	++	++	+	-	-	+w	+	-
38F3-S6	+++	+++	+++	+++	+++	+++	+w	-	-	+	-
37C5-S7	+++	+++	+++	+++	+++	+w	++	+++	++	+w	+
35C6-S11	+++	+++	+++	+++	+++	+w	+++	+	+	+w	+w
35G9-S15A	+++	+++	+++	+++	+++	-	-	-	+w	+w	-
31D4-H12	++	++	++	++	++	-	-	-	+w	+w	-
47G3-S15C	+++	+++	+++	+	+	-	-	+	+	-	-

Results are expressed as ratio of absorbance (A) of a given mutant to that obtained for 1.25 ng/mL of standard recombinant adw HBsAg and presented as : -: ≤ negative control (pRK5 plasmid); +^w: > negative control < 1; +: 1-3; ++: 3-6; +++: > 6.