Differential reactivity of mouse monoclonal anti-HBs antibodies with recombinant mutant HBs antigens

Table 3 Reactivity of mutant HBs antigens secreted by transfected cells as determined by three commercial ELISA kits

Expressed	Bioelisa		ETI-MAK-4		Hepanostika
HBsAg	A450	Index	A450	Index	A450	Index	Conc.
Gly D	> 3	> 5.5	2.9	5.1	> 3	> 11	> 200
Gly Y	2.97	5.5	2.97	5.2	> 3	> 11	> 200
91-4696	> 3	> 5.5	2.87	5	> 3	> 11	> 200
SA6	2.96	5.5	2.74	4.8	> 3	> 11	> 200
SA7	> 3	> 5.5	2.87	5	> 3	> 11	> 200
1056	1.5	2.8	1.8	3.2	2.32	8.6	162
PA17D	1	1.8	2.7	4.7	> 3	> 11	> 200
M5	1.59	2.9	0.74	1.3	1.39	5.1	92
BA2.4	1.66	3.1	2.08	3.6	2.33	8.6	162
SA4	1.67	3.1	1.34	2.3	2.12	7.8	148
BA3.2	0.54	1	0.65	1.1	1	3.7	62
pRK5	0.17	0.31	0.08	0.15	0.1	0.37	0
Medium	0.18	0.33	0.11	0.19	0.1	0.37	0

Results are expressed as absorbance (A) measured at 450 nm, index (ratio of absorbance of mutant sample to absorbance obtained for 1.25 ng/mL of standard adw HBsAg) and concentration (Conc.) presented as ng/mL. Concentration was measured only by the Hepanostika kit using the standard adw HBsAg to construct the standard curve.