Involvement of P53 and Bax/Bad triggering apoptosis in thioacetamide-induced hepatic epithelial cells

Figure 4 A: Morphology of clone 9 cell; (a) untreated (Hoechst 33342 staining); (b) after treatment with 100 mg/L TAA treatment for 16 h (light field); (c) after treatment with 100 mg/L mmol/L TAA for 16 h (Hoechst 33342, 5 mg/mL in PBS). The arrow indicates the apoptotic cells; B: Flow cytometry of apoptosis in clone 9 cells (TUNEL assay) after treatment with 100 mg/L TAA for various times (a) 0 (b) 4 and (c) 8 h. R1 presents the area of cell apoptosis. Individual experiments were repeated three times and each time point of treatment was triplicate.