5-Fluorouracil-related enhancement of adenoviral infection is Coxsackievirus-adenovirus receptor independent and associated with morphological changes in lipid membranes

Figure 5 Increased release of viral particles from liposome-encapsulated adenoviral formulations. 293 cells were infected with the supernatant from centrifuged liposome-entrapped Ad-GFP solutions with and without 5-FU incubation. The number of the infected green cells was counted after 48 h and 72 h infection. The reported data are the average of three experiments and the error bars indicate the standard deviation values (A). On the other hand, the 5-FU treatment of liposome-encapsulated viral DNA did not lead to any DNA release, as confirmed by PCR analysis. Following samples (each loaded in duplicate) were separated by agarose gel electrophoresis: DNA alone (lanes 1 and 2), DNA and 9.6 μmol/L 5-FU (lanes 3 and 4), DNA and lipofectamine (lanes 5 and 6), DNA with lipofectamine and lysis buffer (lanes 7 and 8), DNA with lipofectamine and 5-FU 9.6 µmol/L (lanes 9 and 10) (B). EM images of liposomal preparations. (1) lipofectamine, (2) lipofectamine after treatment overnight with 5-FU, (3) Ad-GFP complexed with lipofectamine, (4) Ad-GFP complexed with lipofectamine after treatment overnight with 5-FU (C).