5-Fluorouracil-related enhancement of adenoviral infection is Coxsackievirus-adenovirus receptor independent and associated with morphological changes in lipid membranes

Figure 1 Effect of 5-FU on the adenoviral infection of colon carcinoma cell lines differing in CAR expression. The drug was used at the dose of 2 μmol/L for the Lovo cells and of 20 μmol/L for the SW480 cells. The virus (Ad-CEAbax) was used at 1 MOI. The cells were treated with 5-FU alone, or infected with the virus in the absence and in the presence of 5-FU (A). The expression of bax with 5-FU and/or the virus was controlled by western blot after 24 h, 36 h and 48 h incubation (B). The expression level of CAR on the colon carcinoma cells line was confirmed by western blot analysis (the expression of actin is reported as a control for loading) (C).