Association of H pylori cagA and vacA genotypes and IL-8 gene polymorphisms with clinical outcome of infection in Iranian patients with gastrointestinal diseases

Table 1 Primer sequences used for detection of cagA gene status and IL-8 or vacA gene polymorphisms

Locus	Primers	Size (bp)
IL-8 -251	Common primer, 5’-tgc ccc ttc act ctg tta ac-3’	336
	A allele, 5’-cca caa ttt ggt gaa tta tca at-3’
	T allele, 5’-cca caa ttt ggt gaa tta tca aa-3’
β-globin	5’- aca caa ctg tgt tca cta gc -3’	100
	5’- caa ctt cat cca cgt tca cc -3’
cagA	5’-aat aca cca acg cct cca ag-3’	400
	5’-ttg ttg ccg ctt ttg ctc tc-3’
vacA
S1 and s2	5’-ctg ctt gaa tgc gcc aaa c-3’	s1 = 259
	5’-atg gaa ata caa caa aca cac-3’	s2 = 286
m1 and m2	5’-gcg tct aaa taa ttc caa gg-3’	m1 = 570
	5’-caa tct gtc caa tca agc gag-3’	m2 = 645
glmM	5’-aag ctt tta ggg gtg tta ggg gtt t-3’	294
	5’-aag ctt act ttc taa cac taa cgc-3’