Administration of granulocyte colony stimulating factor after liver transplantation leads to an increased incidence and severity of ischemic biliary lesions in the rat model

Table 1 Histologic damage score of the liver after liver transplantation

Single cell necrosis	0: No necrotic hepatocyte in 5 HPF (40 X)
	1: 1-10 in 5 HPF
	2: > 10
Confluent necrosis	0
	1: Small in size and number
	2: Large size and /or large number
Hepatic mitosis	0: No mitotic hepatocyte in 5 HPF (40 X)
	1: 1-10 in 5 HPF
	2: > 10 (extensive)
Bile duct inflammation damage	0: No inflammation;
	1: A minority of the ducts are cuffed and infiltrated by inflammatory cells
	2: More than an occasional duct showing degenerative changes or focal degenerative changes; Most of the ducts infiltrated by inflammatory cells
	3: Most of the ducts showing luminal disruption, most of the ducts infiltrated by inflammatory cells
Ductular proliferation	0: None
	1: Minimal (small proliferation in a minority of portal tracts)
	2: Mild (most portal tracts but not involving the lobular parenchyma)
	3: Moderate (all portal tracts and extending along the fibrous septa)
	4: Severe (extending along the portal tracts and also slightly involving the lobular parenchyma)
	5: Very severe (diffuse proliferation in the lobular parenchyma)
Fibrosis	0: None
	1: Fibrosis slightly extending portal tracts
	2: Fibrosis extending portal tracts with incomplete septa
	3: Fibrosis with complete septa bridging portal to portal tracts
	4: Incomplete (focal) or complete cirrhosis
Activated Kupffer cells	0: No activated Kupffer cells
	1: Activated Kupffer cells
Eosinophilic globuli	0: No eosinophilic globuli
	1: 1%-5% of all hepatocytes
	2: > 5%
Small vacuolar transformation of the cytoplasm	0: No small vacuolar transformation of the cytoplasm
	1: 1%-30% of all the hepatocytes
	2: > 30%