DA-9601, a standardized extract of Artemisia asiatica, blocks TNF-α-induced IL-8 and CCL20 production by inhibiting p38 kinase and NF-κB pathways in human gastric epithelial cells

Figure 8 DA-9601 selectively attenuates TNF-α-mediated phosphorylation of p38 kinase in AGS cells. Cells (5 x 10⁵ cells/well) were pretreated for 1 h with medium alone or medium containing DA-9601 (50 μg/mL). Then, the cells were stimulated for 15 min with or without TNF-α (5 ng/mL). The cell lysates were blotted with antibodies specific for the phosphorylated or total forms of ERK1/2, p38 kinase, and JNK/SAPK, and visualized using a peroxidase-conjugated secondary antibody and the ECL system.