Copyright
©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 14, 2006; 12(30): 4850-4858
Published online Aug 14, 2006. doi: 10.3748/wjg.v12.i30.4850
Published online Aug 14, 2006. doi: 10.3748/wjg.v12.i30.4850
Figure 3 DA-9601 blocks IL-8 and CCL20 promoter activities in HEK293T and AGS cells.
HEK293T (left) and AGS (right) cells were transfected with pGL3-pIL-8 (A) or pGL3-pCCL20 (B) luciferase vectors. After 24 h of incubation, cells (1 x 105) were pre-treated for 1 h with DA-9601 (50 μg/mL) and stimulated for additional 16 h with medium alone or medium containing TNF-α (5 ng/mL). At the end of incubation, cells were lysed, and the relative luciferase activity was measured using Luciferase Assay System. Results are expressed as means ± SD of three independent experiments.
- Citation: Choi SC, Choi EJ, Oh HM, Lee S, Lee JK, Lee MS, Shin YI, Choi SJ, Chae JR, Lee KM, Lee WJ, Park JS, Shin CY, Oh TY, Jun CD. DA-9601, a standardized extract of Artemisia asiatica, blocks TNF-α-induced IL-8 and CCL20 production by inhibiting p38 kinase and NF-κB pathways in human gastric epithelial cells. World J Gastroenterol 2006; 12(30): 4850-4858
- URL: https://www.wjgnet.com/1007-9327/full/v12/i30/4850.htm
- DOI: https://dx.doi.org/10.3748/wjg.v12.i30.4850