Cocultivation of umbilical cord blood CD34+ cells with retro-transduced hMSCs leads to effective amplification of long-term culture-initiating cells

Figure 5 PCR analysis of human Alu sequence in NOD/SCID mice 7 wk after transplantation. DNA was extracted from the bone marrow and peripheral blood cells of NOD/SCID mice 7 wk after transplantation. PCR analysis demonstrated the presence of human hematopoietic cells in the bone marrow (lane 2) and peripheral blood cells (lane 3) of NOD/SCID mice. DNA extracted from human UCB cells was used as a positive control (lane 4). Negative control is shown in lane 1 and DNA marker is shown in lane 5.