Cocultivation of umbilical cord blood CD34+ cells with retro-transduced hMSCs leads to effective amplification of long-term culture-initiating cells

Figure 4 Determination of human hematopoietic reconstitution in NOD/SCID mice 7 wk after transplantation. A: Expression of human CD45 on bone marrow cells (BMC) collected from NOD/SCID mice 7 wk after transplantation; (A₁): Cells in the gated area were analyzed by flow cytometry; (A₂): Bone marrow cells (BMC) from an untransplanted mouse (negative control); (A₃): BMC from a mouse transplanted with 1 ×10⁵ human CD34⁺ cells before culture; (A₄): BMC from a mouse transplanted with cells obtained after 14-d cultivation of those 100 000 human CD34⁺ cells in the tfhMSCs coculture system; B₁-B₆: Specific subsets of human CD45⁺ cells in bone marrow cells of the NOD/SCID mouse transplanted with UCB cells after the coculture. Harvested cells were stained with PE-conjugated CD45 and various FITC-conjugated monoclonal antibodies.