Cocultivation of umbilical cord blood CD34+ cells with retro-transduced hMSCs leads to effective amplification of long-term culture-initiating cells

Figure 2 Effects of several culture systems on ex vivo expansion of hematopoietic progenitors. UCB CD34⁺ cells were cultured in serum-containing medium using four culture systems. A: Total nucleated cells; B: CD34⁺ cells; C: Total colony-forming units in culture (CFU-C); D: Mixed colonies containing erythroid and myeloid cells and megakaryocytes (CFU-GEMM). The results represent the mean fold increase ± SD of three different experiments on d 7, 14, and 21 of culture. ¹No significant differences between different culture systems. ^aP < 0.05, ^bP < 0.01 as compared with other three groups. ◆= tfhMSCs coculture system; ▓= hMSCs coculture system; ▲= cytokines culture system; ● = hMSCs (TPO/FL-free) culture system. A = tfhMSCs coculture system; B=hMSCs coculture system; C = cytokines culture system; D = hMSCs (TPO/FL-free) culture system.