Cocultivation of umbilical cord blood CD34+ cells with retro-transduced hMSCs leads to effective amplification of long-term culture-initiating cells

Table 2 Results of LTC-IC assay using UCB CD34+ cells or those generated by four culture systems

Culture systems	LTC-IC frequency		Fold LTC-IC amplification
	Pre-expansion	Post-expansion
hMSCs (TPO/FL-free) culture system	1/619.57 ± 120.89	1/102 258.56 ± 9 568.49	2.79 ± 0.56
Cytokines culture system	1/625.38 ± 154.26	1/84 112.78 ± 10 867.64	3.58 ± 1.48
hMSCs coculture system	1/621.76 ± 138.59	1/93 773.42 ± 20 346.23	5.32 ± 1.73
tfhMSCs coculture system	1/617.43 ± 119.76	1/24 140.96 ± 7 586.62	10.23 ± 2.89b

^bP < 0.01 vs calculated by the Student’s t-test, when data of the tfhMSCs coculture system were compared with data in the other groups.