Growth hormone regulates intestinal ion transport through a modulation of the constitutive nitric oxide synthase-nitric oxide-cAMP pathway

Figure 4 The upper side of the figure shows the cNOS protein expression in Caco-2 cells after 1 h of incubation with GH and CT alone or in combination, as compared to tubulin expression. The cNOS protein expression is revealed by the appearance of 160-kD band that corresponds to human NOS 1 (neuronal NOS). Shown is a representation of 3 separate experiments. In the lower side of the figure an optical densitometry analysis of the bands is also reported.