Cross-species hybridization of woodchuck hepatitis virus-induced hepatocellular carcinoma using human oligonucleotide microarrays

Table 5 RT-PCR and microarray results

Gene	Process	W904		W361		W380
		RT-PCR	Microarray	RT-PCR	Microarray	RT-PCR	Microarray
Testis enhanced gene transcript (BAX inhibitor 1) (TEGT)	Negative regulation of apoptosis	No change	Up	Up	Up	Up	Up
Spermidine/spermine N1-acetyltransferase (SSAT)	Polyamine metabolism	Down	Down	Down	Down	No change	Down
Acetyl-CoA synthetase 2 (thiokinase) (ACAS2)1	Metabolism, lipid biosynthesis	Up	Up	Up	Up	Up	No change
Alpha-fetoprotein (AFP)	Immune response, transport	Up	No change	Up	No change	Up	Up
Isocitrate dehydrogenase 3 (NAD+) beta (IDH3B)	Tricarboxylic acid cycle	Up	Up	Undetermined	No change	Up	Up
Carnitine palmitoyltransferase II (CPT2)	Lipid metabolism, fatty acid metabolism, transport	Up	No change	Up	No change	No change	No change
N-myc 2 (NMYC2)	Unknown	Undetermined	N/A	Up	N/A	Undetermined	N/A
C-myc (CMYC)2	Regulation of transcription, DNA-dependent	Up	Up	Down	Up	No change	No change
Fatty-acid-Coenzyme A ligase, very long-chain 1 (FACVL1)	Lipid metabolism, fatty acid metabolism	Down	Down	Undetermined	Down	Up	No change
Hexokinase (GCKR)3	Carbohydrate metabolism	Undetermined	No change	Undetermined	Up	Up	No change

¹RT-PCR results are for ACAS2, microarray results are for a hypothetical protein similar to ACAS2.

²RT-PCR results are for c-myc, microarray results are for a c-myc binding protein and a downstream target.

³RT-PCR results are for glucokinase regulatory protein, microarray results are for an EST similar to hexokinase domain containing 1.