Cross-species hybridization of woodchuck hepatitis virus-induced hepatocellular carcinoma using human oligonucleotide microarrays

Table 1 RT-PCR primer and probe sequences for select genes

Target gene	Forward primer sequence	Reverse primer sequence	Reporter probe sequence
Testis enhanced gene transcript (BAX inhibitor 1) (TEGT)	GCCACCTGTAATGTGTGCAAAG	ACTTTCCCAGTTTGACTTCCTCTTC	CTCCACCCCTCATGTCC
Acetyl-CoA synthetase 2 (thiokinase) (ACAS2)	TGCTGAGGACCCACTCTTCAT	AGCATGTAACCGCCAACTGT	CCACAGGCAAACCCA
Alpha-fetoprotein (AFP)	AGGCTGTCATTGCAGATTTCTCT	GGACCCTCTTCTGCAAAGCA	CTGGCAGCATGTCTCC
C-myc (CMYC)	CAGAAGGTCAGAATCGGTGTCA	AGGACCAGTGGGCTGTGA	CCACAGCAAACCTC
Hexokinase (glucokinase regulatory protein) (GCKR)	ATGCTGCAGCGGTTCTCT	GGATCGCTTGGAGGAGACTCT	AAGGCCCGATGCATTG
Isocitrate dehydrogenase 3 (NAD+) beta (IDH3B)	TCTCAGCGGATTGCAAAGTTTG	CTTGTGGACAGCTGTGACCTT	CCCGCCCCTTCTTG
Fatty-acid-Coenzyme A ligase, very long-chain 1 (FACVL1)	GCGGGATGACACAGCAAAA	TCTTTCTGGAAATGTGAGTTATCCTTCTG	TCTGAAAATCTGAATATCCC
Spermidine/spermine N1-acetyltransferase (SSAT)	TTTCATGCAACACTTGGTCTCTCT	CACTGGACTCCGGAAGGTAAC	CCCTCACCCAATCCAG
Carnitine palmitoyltransferase II (CPT2)	TGCCTATTCCCAAACTTGAAGACA	TCTTCCTGAACTGGCCATCATTC	TCAATGCACAGAAACCT
N-myc 2 (NMYC2)	GGACGCGCTGAGTGGAT	GCTCTCGTTCGGCTCCAA	TTCCCAGGAGACCCC