Mechanism of T cell hyporesponsiveness to HBcAg is associated with regulatory T cells in chronic hepatitis B

Figure 2 FACS analysis of HBcAg-specific production of IL-10 in patients with hepatitis B. Cellular source of HBcAg-specific production of IL-10 was identified by staining for IL-10-secretion (PE-labeled), anti-CD3-PerCP, anti-CD4-PerCP and anti-CD25-FITC. Representative dot plots of IL-10-secreting CD4⁺ T cells in a patient with CHB (A) and IL-10-secreting CD4⁺CD25^high T cells in a patient with CHB (B). For the control, IL-10-secreting cells in a healthy subject with HBcAg stimulation (C) and in a patient with CHB with HBsAg stimulation (D) were also shown. Numbers shown in the dot plots indicate percentage of the cells in the quadrant region.