Amplification of D22S283 as a favorable prognostic indicator in liver fluke related cholangiocarcinoma

Table 2 Sequences of markers located at chromosomal region 22q12-qter including two housekeeping genes

Gene/marker	Band	Primer sequence (5’-3’)
D22S283	22q12.3	5’ ACC AAC CAG CAT CAT CAT 3’ 5’ AGC TCG GGA CTT TCT GAG 3’
D22S423	22q13.1-13.2	5’ TGC AAA CTC AGC CTG GA 3’ 5’ ACC AAC TGA CTC GTT TAG GTC AT 3’
D22S274	22q13.3	5’ GTC CAG GAG GTT GAT GC 3’ 5’ AGT GCC CAT TTC TCA AAA TA 3’
NF2	22q12.2	5’ AAG AGC AAG CAT CTG CAG GA 3’ 5’ TGG TAT TGT GCT TGC TGC TG 3’
TIMP3	22q12.3	5’ TGT CTC TGG ACC GAC ATG CT 3’ 5’ TGG CGC TCA GGG ATC TGT G 3’
ST13	22q13.2	5’ GTT ACA CTA TTT AAG AGC TGA AT 3’ 5’ GGT CTT CTA CTT AGA AAA ACC TA 3’
TOB2	22q13.2-q13.31	5’ GAA GAC ACC CCT TTG TGG AA 3’ 5’ TCT GTG GTC TTG GGT GCT C 3’
BIK	22q13.31	5’ ATG ACC ACT GCC CTG GAG 3’ 5’ CTA AAC ACA GGC CAC AGT TAA CC 3’
TP	22q13.33	5’ GGG GCT CAA GTC GCG AGG 3’ 5’ CCT GCG GGG ATG CCT GAC 3’
β-actin (Reference)	7p15-p12	5’ TCA CCC ACA CTG TGC CCA TCT ACG A 3’ 5’ CAG CGG AAC CGC TCA TTG CCA ATG G 3’
GAPDH (Reference)	12p13	5’ ACA GTC CAT GCC ATC ACT GCC 3’ 5’ GCC TGC TTC ACC ACC TTC TTG 3’