Antioxidant role of heme oxygenase-1 in prehepatic portal hypertensive rats

Table 3 Effect of Sn-PPIX administration on the HO-1 induction and on TBARS and GSH contents in PPVL rat livers (mean ± SE)

Treatment	HO-1	TBARS	GSH content
	(U/mg protein)1	(nmol/min per mgprotein)	(μmol/g liver)
Sham	0.50 ± 0.01b	0.10 ± 0.01d	7.5 ± 0.2d
PPVL	0.90 ± 0.02	0.25 ± 0.01	3.7 ± 0.1
Sham + Sn-PPIX	0.25 ± 0.01	0.11 ± 0.01d	7.2 ± 0.3d
PPVL + Sn-PPIX	0.48 ± 0.04	0.32 ± 0.02	2.3 ± 0.2

Sn-PPIX was administered as described in Materials and Methods. Sham animals were injected with saline solution. Enzymatic activity was assayed as described in the text.

¹ One unit of the enzyme forms 1 nmol of bilirubin/30 min under assay conditions. Different letters within columns indicate significant differences according to Neuman-Keuls' test. Differences in HO-1 were of

^bP < 0.01 between Sham group and PPVL, Sham + Sn-PPIX groups. Differences in TBARS and in GSH content were of

^dP < 0.01 between Sham groups and PPVL groups.