Antioxidant role of heme oxygenase-1 in prehepatic portal hypertensive rats

Table 2 Effect of PPVL and bilirubin on antioxidant enzyme activities (mean ± SE)

Group	Catalase (pmol/mg protein)	Total superoxidedismutase (U/mg protein)1	Glutathione peroxidase (U/mg protein)2
Sham	10.5 ± 1.1a	9.0 ± 0.2b	0.129 ± 0.004a
PPVL	6.5 ± 0.5	4.5 ± 0.1	0.100 ± 0.004
Sham + Bilirubin	10.7 ± 0.9a	9.3 ± 0.1b	0.132 ± 0.006a
PPVL + Bilirubin	9.4 ± 0.9a	8.9 ± 0.2b	0.131 ± 0.004a

Enzymatic activities were assayed as described in the text.

¹ One unit of SOD activity is defined as the amount of enzyme required to inhibit 50% of the epinephrine autooxidation.

² One unit of the enzyme represents the decrease of 1 mmol of NADPH/min under the assay conditions.

^aP < 0.05 between Catalase and Glutathione Peroxidase of Sham groups, PPVL + Bilirubin and PPVL group,

^bP < 0.01 between Superoxide Dismutase of group of Sham groups, PPVL + Bilirubin and PPVL group and PPVL group according to Neuman-Keuls' test.