Amplification of chromosome 21q22.3 harboring trefoil factor family genes in liver fluke related cholangiocarcinoma is associated with poor prognosis

Table 3 Univariate and multivariate analysis of overall survival in cholangiocarcinoma

Variable	n	Univariate		Multivariate
		Relative risk(95%CI)	P value	Relative risk(95%CI)	P value
Age (yr)	69	0.831 (0.483-1.430)	NS
≤ 54	34
＞ 54	35
Gender	69	0.443 (0.231-0.849)	0.014	0.628 (0.312-1.263)	NS
Male	49
Famale	20
Histological type	69	0.622 (0.332-1.167)	NS
Non-Papillary adenocarcinoma	50
Papillary adenocarcinoma	19
Staging	69	2.265 (0.899-5.708)	0.083	3.320 (1.270-8.681)	0.014
II & III	9
IVA & IVB	60
Blood vessel invasion	69	2.108 (1.139-3.902)	0.018	2.183 (1.088-4.382)	0.028
Non-invasion	23
Invasion	46
Lymphatic invasion	69	0.981 (0.479-2.012)	NS
Non-invasion	12
Invasion	57
Nerve invasion	69	1.129 (0.654-1.950)	NS
Non-invasion	32
Invasion	37
D21S1893, D21S1890, and TFF	69		0.026		0.002
Normal	30	Reference		Reference
Amplification	35	1.707 (0.974-2.991)	NS	2.473 (1.342-4.557)	0.004
Deletion	4	0.187 (0.025-1.394)	NS	0.224 (0.029-1.701)	NS