Amplification of chromosome 21q22.3 harboring trefoil factor family genes in liver fluke related cholangiocarcinoma is associated with poor prognosis

Table 2 Clinicopathological parameters of patients with and without allelic imbalance

Clinical parameters	n	Gene/microsatellite makersD21S1893, D21S1890 and TFF
		Normaln (%)	Amplificationn (%)	Deletionln (%)	P value
Age (yr)	80
≤ 54	39	18 (46)	20 (51)	1 (3)	NS
＞ 54	41	15 (36)	22 (54)	4 (10)
Gender	80
Male	57	22 (39)	32 (56)	3 (5)	NS
Famale	23	11 (48)	10 (43)	2 (9)
Histological type	80
Non-Papillary adenocarcinoma	58	27 (46)	30 (52)	1 (2)	0.023
Papillary adenocarcinoma	22	6 (27)	12 (55)	4 (18)
Staging	80
II & III	10	5 (50)	5 (50)	-	NS
IVA & IVB	70	28 (40)	37 (52.8)	5 (7.2)
Blood vessel invasion	79
Non-invasion	28	8 (29)	16 (57)	4 (14)	NS
Invasion	51	24 (47)	26 (51)	1 (2)
Lymphatic invasion	79
Non-invasion	15	4 (27)	11 (73)	-	NS
Invasion	64	28 (43)	31 (49)	5 (8)
Nerve invasion	79
Non-invasion	36	14 (39)	19 (53)	3 (8)	NS
Invasion	43	18 (42)	23 (54)	2 (4)
Survival time (wk)	69				0.012
Mean		54.12	51.7	124.82
Median		49	27.4	84.28
Minimum-maximum		7.14-119.28	4.85-242.85	67.71-143.85