Depression of biofilm formation and antibiotic resistance by sarA disruption in Staphylococcus epidermidis

doi:10.3748/wjg.v12.i25.4009

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Jul 7, 2006 (publication date) through Jul 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jul 7, 2006; 12(25): 4009-4013
Published online Jul 7, 2006. doi: 10.3748/wjg.v12.i25.4009

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Figure 1 Disruption and detection of chromosomal sarA locus in S. epidermidis. 1. Insertion of tet gene into sarA locus to form recombinant plasmid pBT△sarA. 2. Double-exchange homologous recombination between chromosomal DNA and plasmid DNA. 3. Locus sarA of chromosome was destroyed by DNA recombination. 4. Detection of recombination by PCR amplification with primers TB and SA2.

Citation: Tao JH, Fan CS, Gao SE, Wang HJ, Liang GX, Zhang Q. Depression of biofilm formation and antibiotic resistance by sarA disruption in Staphylococcus epidermidis. World J Gastroenterol 2006; 12(25): 4009-4013
URL: https://www.wjgnet.com/1007-9327/full/v12/i25/4009.htm
DOI: https://dx.doi.org/10.3748/wjg.v12.i25.4009