Interaction between enteric epithelial cells and Peyer’s patch lymphocytes in response to Shigella lipopolysaccharide: Effect on nitric oxide and IL-6 release

Figure 5 Comparison of mIL-6 release induced by Shigella LPS in various Caco-2 and PPL culture configurations. Shigella LPS was added to four different cultures (set 1: wild-type PPL only; set 2: Caco-2 and wild-type PPL coculture; set 3: iNOS knockout PPL only; set 4: Caco-2 and iNOS knockout PPL coculture) and the level of mIL-6 release was measured by mIL-6 ELISA and the values were derived based on a standard curve. The black bar and the gray bar represent 24 h treatment with and without LPS, respectively. The values represent the mean ± SE for 4 different samples; ^bP < 0.001 vs its own untreatment group, ^dP < 0.001 vs wild-type PPL 24-h after challenge Shigella LPS and ^fP < 0.001 vs wild-type coculture 24-h after Shigella LPS challenge.