High efficient mammalian expression and secretion of a functional humanized single-chain Fv/human interleukin-2 molecules

Figure 6 Anti-p185 mAb, 520C9, specifically blocks the binding of H520C9sFv hIL 2 fusion protein to SKOV 3ip1 cells. SKOV 3ip1 cells, pre-exposed to serially diluted intact 520C9 monoclonal antibody or to mouse IgG (0.0001 to 10 nmol/L), then incubated with 10 nmol/L H520C9sFv hIL 2 fusion protein. Bound fusion protein was measured by indirect ELISA using the anti human IL 2 polyclonal antibody, EP100. 520C9 mAb caused a dose-dependent decrease in the binding of the fusion protein to SKOV 3ip1 cells. Control mouse IgG had no effect on the binding of the fusion protein. The data are the mean ± SD from three separate experiments. ^bP < 0.01 vs mouse IgG, P > 0.05 vs mouse IgG.