High efficient mammalian expression and secretion of a functional humanized single-chain Fv/human interleukin-2 molecules

Figure 2 IL 2 activity of recombinant humanized sFv antibody/IL-2 fusion proteins. IL 2 activity of rhIL-2, or conditioned medium from 293 cells transfected with either pcDNA-H520C9sFv hIL 2 or pcDNA-H520C9sFv mhIL 2 was measured by [³H] thymidine incorporation in CTLL 2 cells. The data are the mean ± SD from three separate experiments. ^bP < 0.01 for rhIL-2 vs H520C9sFv mhIL 2, and P > 0.05 vs H520C9sFv hIL 2; ^aP < 0.05 for rhIL-2 vs H520C9sFv mhIL 2, and P > 0.05 vs H520C9sFv hIL 2.