Single-tube-genotyping of gastric cancer related SNPs by directly using whole blood and paper-dried blood as starting materials

doi:10.3748/wjg.v12.i24.3814

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Jun 28, 2006 (publication date) through Nov 5, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Gastric Cancer

World J Gastroenterol. Jun 28, 2006; 12(24): 3814-3820
Published online Jun 28, 2006. doi: 10.3748/wjg.v12.i24.3814

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Figure 3 Direct PCR with two sets of primers (P1-1 and P1-2; P2-1 and P2-2) using 0. 5 μL (a1, b1), 1 μL (a2, b2), 2 μL (a3, b3) and 4 μL (a4, b4) of EDTA-blood dried on filter paper as starting materials. PCR with 1 μL of fresh blood without an anticoagulant was performed in parallel for the comparison (a5, b5). a1-a5: 379-bp fragment; b1-b5: 539-bp fragment.

Citation: Huang H, Bu Y, Zhou GH. Single-tube-genotyping of gastric cancer related SNPs by directly using whole blood and paper-dried blood as starting materials. World J Gastroenterol 2006; 12(24): 3814-3820
URL: https://www.wjgnet.com/1007-9327/full/v12/i24/3814.htm
DOI: https://dx.doi.org/10.3748/wjg.v12.i24.3814