Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-κB activation

Table 4 Real-time quantification of mRNA levels of several genes in HT-29 cells

Treatment	Relative mRNA level 1± SD of gene for
	IL-8	TNF-α	COX-2	ICAM-1
LPS+HM (positive control)	40.6 ± 17.4	37.4 ± 12.6	5.9 ± 1.7	28.0 ± 10.7
B. longum NCC2705/ LPS+HM 2	11.4 ± 1.1 a	12.7 ± 1.8 a	2.5 ± 1.6	12.2 ± 9.2
B. bifidum S17/ LPS+HM 2	3.5 ± 2.6 a	2.9 ± 2.4 a	1.4 ± 0.6 a	2.0 ± 0.8  a

¹ Results are mRNA levels relative to the negative control (no bacteria, no LPS) and are mean ± standard deviation (SD) of three independent pools of RNA for each condition.

² Pre-incubation with B. longum NCC2705 or B. bifidum S17 at moi = 100 was followed by stimulation with LPS + HM for 4 h.

^aP < 0.05 vs positive control; Student’s t-test.