Activation and dramatically increased cytolytic activity of tumor specific T lymphocytes after radio-frequency ablation in patients with hepatocellular carcinoma and colorectal liver metastases

Table 1 Stimulation indices (SI) of each patient before, 4 and 8 wk after RFA treatment

Tumor entity	CP	Etiology ofcirrhosis	SI before		SI 4 wk		SI 8 wk
			RFA		after RFA		after RFA
			CD4	CD8	CD4	CD8	CD4	CD8
HCC 1	A	alcohol	0.012	0.017	0.086	0.125	0.15	0.146
HCC 2	A	Hep C	0.021	0.019	0.16	0.138	0.176	0.136
HCC 3	B	Hep C	0.016	0.031	0.11	0.102	0.155	0.158
HCC 4	A	alcohol	0.015	0.019	0.07	0.11	0.079	0.112
HCC 5	B	alcohol	0.026	0.021	0.069	0.118	0.099	0.139
HCC 6	A	alcohol	0.02	0.02	0.16	0.125	0.146	0.134
Mean (SD)			0.018 (± 0.005)	0.021 (± 0.005)	0.109b (± 0.042)	0.120b (± 0.012)	0.134b (± 0.037)	0.138b (± 0.015)
Tumor entity	Primary		SI before		SI 4 wk		SI 8 wk
			RFA		after RFA		after RFA
			CD4	CD8	CD4	CD8	CD4	CD8
CRC metastasis 1	Sigma		0.025	0.023	0.123	0.158	0.129	0.155
CRC metastasis 2	Colon		0.035	0.029	0.155	0.176	0.199	0.143
CRC metastasis 3	Colon		0.01	0.017	0.069	0.11	0.126	0.125
CRC metastasis 4	Sigma		0.016	0.018	0.099	0.127	0.112	0.126
CRC metastasis 5	Rectum		0.011	0.02	0.103	0.183	0.175	0.176
CRC metastasis 6	Colon		0.02	0.021	0.14	0.147	0.149	0.137
Mean (SD)			0.019 (± 0.009)	0.021 (± 0.004)	0.115b (± 0.031)	0.150b (± 0.028)	0.148b (± 0.033)	0.144b (± 0.019)

CP: Child Pugh-Score of patients with liver cirrhosis; LC: liver cirrhosis; SI: Stimulation index, showing the ratio of IFNγ secretion of stimulated vs unstimulated T cells; HCC: hepatocellular carcinoma ; CRC metastasis: patients with colorectal cancer metastatic to the liver; Primary: location of the primary tumor; m.d.: missing data.

^bP < 0.0001 vs SI before RFA.