Gene expression profiling defined pathways correlated with fibroblast cell proliferation induced by Opisthorchis viverrini excretory/secretory product

Table 1 List of signal transduction genes¹ validated the expression by real-time RT-PCR, their corresponding PCR primers², annealing temperature and product sizes

Group of stimulating Gene growth factor		Primer sequence	Annealing temperature (°C)/ product size (bp)
PDGF/EGF	abl 1	Forward: 5’-TGAAGTTGGTGGGCTGCA-3’	52/148
		Reverse: 5’-TTTTCACTGGGCCCGCA-3’
	pkC	Forward: 5’-GGCATGCCTTGTCCTGGAGA-3’	55/149
		Reverse: 5’-TGTAGCCCTGCCTCGAGAGA-3’
	jak 1	Forward: 5’-CAGCCTCGCTCGTCCTT-3’	58/147
		Reverse: 5’-CCAAGCCCTTCAGAGCT-3’
	raf 1	Forward: 5’-TTTCCCGGATGCCTGCTA-3’	62/147
		Reverse: 5’-TCCTGCTGTCCCATGCA-3’
	h ras	Forward: 5’-TGGTGGGCAACAAGTGTGA-3’	65/150
		Reverse: 5’-CCGCAATTTATGCTGCCGAA
PDGF	pdgfrα	Forward: 5’-TGCGGGGAAGGACTGGA-3’	58/122
		Reverse: 5’-GTGAGGAGACAGCTGAGGA-3’
EGF	eps 8	Forward: 5’-CCGCTCCGTGGGTATGGA-3’	54/146
		Reverse: 5’-ACGTCCGACACACTGCTGA-3’
TGF-β	tgfβ 1i4	Forward: 5’-GCAAGTGTGGTAGCTATCGA-3’	52/136
		Reverse: 5’-CAGATTGTTCTCCTGCTCC-3’
	strap	Forward: 5’-GCCGGGAGATACAGGAGA-3’	52/153
		Reverse: 5’-TGCTTATGAGCCGGGTCA-3’

¹They are classified based on their functions as the downstream signaling molecules of the specific growth factor. ²Primers for the internal control (β₂m) are: 5’-CATGGCTCGCTCGGTGA-3’ (forward) and 5’-AATGTGAGGCGGGTGGAA-3’ (reverse). The annealing temperature and the product size of RT-PCR for β₂m are 60°C and 148 bp, respectively. PDGF: platelet-derived growth factor; EGF: epidermal growth factor; TGF-β: transforming growth factor-β.